Sucrose flux estimated from the initial kinetics of a 0–5 min 13Ci-VHC pulse.
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Each replicate experiment (WT n = 3, mutants n = 2) was an independent time series of 7–8 time points sampled ∼0.5–60 min after the pulse. The sucrose flux was calculated based on the influx of carbon from the G6P pool according to previously described methods [39].Cultures were pre-acclimated to 5% CO2 (HC) or 0.035% CO2 (LC) of ambient isotope composition.
创建时间:
2015-12-02



