Zinc Uptake and Storage During the Formation of the Cerebral Cortex in Mice

The aim of this work was to analyse the transcriptome of the mice cerebral cortex (or neocortex) during embryogenesis and at post-natal age. This complete transcriptomic analysis was dedicated to the analysis of the genes participating in the homeostasis of Zinc during brain development and formation. Our study represents a detailed analysis of transcriptomes during formation and dvelopment of the brain cortex, with biologic triplicates, generated by RNA-seq technology. This will provide a framework for comparative investigations of expression profiles. Our work offers an accurate quantitative and qualitative evaluation of mRNA content during mice embryogenesis. Total mRNA from cerebral cortices of C57Bl6/J mice were generated in triplicate. Tissues were collected at embryonic days 11 (E11), E13, E17 and on post-natal day 1 pups.本研究旨在分析小鼠大脑皮层（cerebral cortex，又称新皮层neocortex）在胚胎发生（embryogenesis）期与出生后阶段的转录组（transcriptome）。本次完整的转录组分析（transcriptomic analysis）聚焦于参与脑发育与形成过程中锌（Zinc）稳态（homeostasis）调控的相关基因。本研究对大脑皮层形成与发育过程中的转录组开展了详细解析，实验设置了三次生物学重复（biologic triplicates），转录组数据通过RNA测序（RNA-seq）技术生成。本研究可为基因表达谱（expression profiles）的比较分析提供参考框架。本研究可实现对小鼠胚胎发生过程中信使RNA（mRNA）含量的精准定量与定性评估。我们通过三次生物学重复制备了C57Bl6/J小鼠大脑皮层的总mRNA。组织样本分别采集于胚胎第11天（E11）、E13、E17以及出生后第1天的幼崽。