Pd1 regulates proliferation and differentiation of neural progenitors during brain development
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To gain a total insight into how Wt1 regulates embryonic neurogenesis, RNA-seq was performed to compare the different expressed genes at E13. Approximately approximately one thousand transcripts showed differential expression between the Pd1fl/fl and Pd1CKO mice brain cortex, with a fold change >=1.5 and p value <0.05. These results indicated the importance of Pd1 in cortical development. RNA-seq based transcriptome characterization would provide a global understanding how Pd1 gene contribute to brain cortical development. Total RNA were extracted from E13 telencephalic tissue of 2 Pd1fl/fl and 2 Pd1CKO mice. Then the total RNA was quality controlled and quantified using an Agilent 2100 Bioanalyzer. After converting to cDNA and building library, high-throughput sequencing was performed using the Ptc 225 Peltier Thermal Cycler.
