Imaging dataset for positron emission microscopy of patient-derived tumor organoids

Here, we present a microscopy method to image 18F-fluorodeoxyglucose and other radiotracers in patient-derived tumor organoids with spatial resolution up to 100-fold better than that of clinical positron emission tomography (PET). When combined with brightfield imaging, this metabolic imaging approach functionally mirrors clinical PET/CT scans and provides a quantitative readout of cell glycolysis other metabolic processes. Methods The dataset was collected in a custom-built radioluminescence microscope setup. The images are collected by an EMCCD (Hamamatsu). The images are raw and unprocessed.