Extensive Functional Redundancy of Mammalian Enhancers [RNA-seq]

Analysis of the transcriptional changes in the limb resulting from the loss Gli3 limb enhancers Forelimbs were dissected from wild-type, single and double and enhancer-null embryos and processed for RNA-seq analysis.本研究针对Gli3肢体增强子缺失导致的肢体转录变化开展分析。实验过程中，研究人员从野生型、单突变体、双突变体及增强子完全缺失（enhancer-null）的胚胎中分离前肢，随后进行RNA测序（RNA-seq）分析。