<strong>Hepatic lipidomics in mice with early-life antibiotic exposure and metabolic dysfunction-associated fatty liver disease (MAFLD)</strong>

Using an early-life antibiotic exposure model in BALB/c mice, we characterized the impact of prenatal and/or postnatal antibiotic exposures on the development of MAFLD in offspring through maternal administration of penicillin V. Pups were assigned into the four groups per their antibiotic exposure status. 5 pups born by dams with both prenatal and postnatal antibiotic exposure were grouped as AA group; 6 pups born by dams with only prenatal antibiotic exposure were grouped as AC group; 6 pups born by dams with only postnatal antibiotic exposure were grouped as CA group; 6 pups born by dams without maternal antibiotic exposure were grouped as CC group. All groups of pups underwent a 10% kcal fat standard chow diet period for five weeks before shifting to a 45% kcal high-fat diet for 15 weeks. Mice liver were harvested at the end of the experiment for nontargeted lipidomics. 25 mg of mice liver was weighted, mixed with 800 μL of dichloromethane: methanol (3:1, v/v) and homogenized for lipid extraction. The homogenate was centrifuged and the supernatant was taken for lyophilization and reconstitution. A LC-MS system consisting of Waters 2D UPLC (waters, USA) and Q Exactive high resolution mass spectrometer (Thermo Fisher Scientific, USA). Untargeted lipidomics data is deposited in this repository.