Genome-wide characterization of the transcriptional program of Myc-dependent transformation, expression study. Homo sapiens

We profiled Myc binding in a normal breast cell-line (MCF10A) under basal conditions after ectopic expression of Myc. We showed that ectopic Myc expression increases tumour formation in vivo and in vitro. We then profiled genome-wide Myc binding using Agilent promoter arrays in MCF10A cells expressing wild-type and ectopic Myc. We show that Myc binds to a greater number of spots in wild-type than in Myc cells, but that some targets are unique to each condition. Keywords: genetic-modification and stress-response Overall design: We compared ectopic Myc to wild-type cells under wild-type and serum-deprived conditions with 4 replicates per condition