Arabidopsis thaliana Raw sequence reads. Arabidopsis thaliana
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The off-target efficiency of transgenic lines containing inducible CRISPR/Cas9 system
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2018-09-24
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Cut_efficiency_and_off_target_cleavage_analysis_of_the_CRISPR_Cas9_system. Cut_efficiency_and_off_target_cleavage_analysis_of_the_CRISPR_Cas9_system
The CRISPR/Cas9 is a new system that can introduce site-specific double strand breaks. In this system, sequence specificity is achieved by 20-nucleotide RNA molecules that guild endonuclease to the sp
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Additional file 1: of Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana
Table S1. gRNA candidates for IR and C1 of CLCuMuV. (XLSX 12 kb)
Figshare2019-03-06 更新30
Data_Sheet_13_CRISPR-Cas9 Targeting of the eIF4E1 Gene Extends the Potato Virus Y Resistance Spectrum of the Solanum tuberosum L. cv. Desirée.PDF
Translation initiation factors and, in particular, the eIF4E family are the primary source of recessive resistance to potyviruses in many plant species. However, no eIF4E-mediated resistance to this v
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A multi-purpose toolkit to enable advanced genome engineering in plants
Using our modular cloning system, we constructed 5 vectors targeting two sites in tomato ARF8A gene using two gRNAs to compare the efficiency of mutation induction using six different gRNA expression
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Data_Sheet_8_CRISPR/Cas9-Mediated Targeted Mutagenesis of CYP93E2 Modulates the Triterpene Saponin Biosynthesis in Medicago truncatula.PDF
In the Medicago genus, triterpene saponins are a group of bioactive compounds extensively studied for their different biological and pharmaceutical properties. In this work, the CRISPR/Cas9-based appr
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