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EC-catalytic subunit dependent erythroid precursor transcriptome. EC-catalytic subunit dependent erythroid precursor transcriptome

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NIAID Data Ecosystem2026-03-12 收录
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We tested whether Dis3 and Exosc10 depletion impact erythropoiesis by interfering with RNA processing and generating an aberrant transcriptome. Overall design: For genome-wide transcriptomic analysis, GFP+ erythroid precursors (CD71intTer119-) and proerythroblasts (CD71int/+Ter119-) were purified from firefly luciferase, Dis3 or Exosc10 shRNA-expressing fetal liver cells. RNA-seq was conducted in quadruplicate with RNA isolated from cells sorted 2 days after culture in erythroid expansion media.

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2021-02-19
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