Calculated Max and EC50 Responses of Normal GSF Cell Lines.

Values calculated by applying the 4-parameter curve fit algorithm available in SigmaPlot to multiple replicate experiments. For individual cell lines, all data are presented as absolute numerical measurements. For the average normal response, nuclear translocation is reported as the percentage point change from untreated samples whereas hyperspeckling and total AR is reported as fold change from untreated samples. Calculated maximal responses from AR HCA. EC50 values for Nuclear Translocation, Hyperspeckling and Total AR increase after stimulation with three AR agonists (DHT, Mibolerone and R1881) in GSF from 6 normal individuals (M6382, L7728, M7118, A4676, S8558, B8906). EC50 represents the nM concentration of agonist that provokes a response halfway between the baseline and maximal induction. Maximal response achieved with each agonist for each HTM parameter is expressed as % signal present in the nucleus for Nuclear Translocation, as fold-induction from time point 0 for Hyperspeckling and Total AR content.