Construction of A Human Cell Landscape by Single-cell mRNA-seq

We used single-cell RNA sequencing to determine the cell type composition of all major human organs and construct a basic scheme for the human cell landscape (HCL). We reveal a single-cell hierarchy for many tissues that have not been well characterized previously. We present a "single-cell HCL analysis" pipeline that accurately defines human cell types and exemplify its utility in stem cell biology. Finally, we perform single-cell comparative analysis of the human and mouse cell atlases to reveal the conserved genetic networks in the mammalian system. Microwell-seq analyses of 60 types of human tissues and supplementary datasets for the Mouse Cell Atlas data.本研究采用单细胞RNA测序（single-cell RNA sequencing）技术，明确了人体所有主要器官的细胞类型组成，并构建了人类细胞景观（human cell landscape, HCL）的基础框架。本研究揭示了此前尚未被充分解析的多种组织的单细胞层级结构。我们提出了一套可精准鉴定人类细胞类型的“单细胞HCL分析”流程，并以干细胞生物学领域的应用场景验证了其实用价值。最后，本研究对人类与小鼠细胞图谱开展单细胞对比分析，揭示了哺乳动物系统中保守的遗传调控网络。本研究通过微孔测序（Microwell-seq）技术对60类人体组织进行了分析，并补充了小鼠细胞图谱（Mouse Cell Atlas）的配套数据集。