Tn-seq banks for each WT and Sde family mutant strain were generated. WT and the mutant strain have 15 and 14 banks, respectively, and each bank was barcoded separately and subjected to deep sequencin
Characterization of phosphonate transporter coded by the phnCDE genes as a inorganic phosphate transporter. The reversion of the 8 bp insertion with the ORF of phnE of E. coli K12 MG1655 rendered the