WT and N88S seipin-expressing cells upon bathophenanthrolinedisulfonate (BPS) treatment

WT and N88S seipin-expressing cells were grown in SC-glucose medium to exponential phase and then iron chelator bathophenanthrolinedisulfonate (BPS) was added to a final concentration of 100 micromolar. Cells were incubated in the presence of BPS for an additional 4 hour period. We intend to evaluate differentially expressed genes (DEGs) between the two strains. Four biological replicates (classified as 1-4 for the corresponding strain) were used per strain. Overall design: Cells were treated with BPS to induce iron starvation conditions and evaluate differentially expressed genes (DEGs): WT (G1, 4 biological replicates) vs N88S (G2, 4 biological replicates). 2024 BGI Genomics Co.,Ltd.