Proteomic analysis of senescent translatome

To examine senescent translatome, we combined L-azidohomoalanine (AHA)-mediated labeling of newly synthesized proteins followed by click chemistry for biotin conjugation with the DiDBiT in proliferating and senescent cells. Senescence was induced using a stress-induced senescence model (bleomycin-induced senescence). We further examined the effect of EIF4H-S knockdown on the translatome using a shRNA targeting EIF4H-S.