Transcription profiling of wildtype or Hyp (X-linked hypophosphatemic) mouse kidney after feeding control or low phosphate diet to study changes in gene expression in response to low-phosphate diet

This is a study of the change in gene expression in mouse kidney after feeding control (1.0% P) or low phosphate diet (0.03% P) for 3 or 5 days to normal or Hyp (X-linked hypophosphatemic) mice at 5 weeks of age. The mice were C57BL/6J. Normal wild-type mice, hemizygous Hyp (Hyp/Y) male mice, or heterozygous (Hyp/+) female mice were used. The gene is dominant, so that the two genders are equally affected with low renal retention of phosphate and severity of their bone disease. RNA from 3 mice were pooled for each microarray. The arrays were processed as described in the Affymetrix GeneChip Expression Analysis Technical Manual (copyright 2001, Affymetrix, Inc., Santa Clara, CA), rev. 1, Part number 701021. The data for each array were scaled to an average signal value of 500 for all genes on the array.本研究针对5周龄正常小鼠与Hyp（X连锁低磷血症，X-linked hypophosphatemic）小鼠，分别饲喂对照日粮（磷含量1.0%）或低磷日粮（磷含量0.03%）3天或5天后的肾脏基因表达变化展开探究。实验所用小鼠品系为C57BL/6J，实验对象包括正常野生型小鼠、半合子Hyp雄性小鼠（Hyp/Y）以及杂合子Hyp雌性小鼠（Hyp/+）。该致病基因为显性遗传，因此雌雄小鼠均会出现同等程度的肾脏磷重吸收降低，且骨病变严重程度一致。每个基因芯片（microarray）样本均混合3只小鼠的总RNA进行制备，芯片实验流程严格参照《Affymetrix GeneChip表达分析技术手册》（2001年版权所有，Affymetrix公司，美国加利福尼亚州圣克拉拉市）第1修订版，部件编号701021执行。所有芯片的原始数据均经过标准化处理，使芯片上所有基因的平均信号值为500。