Activation of Hydroxy-carboxylic Acid Receptor 1 effect on cardiomyocytes

Neonatal rat cardiomyocytes (NRCMs) were isolated and cultured. Subsequently, NRCMs were treated with the agonist of Hydroxy-carboxylic Acid Receptor 1 (HCAR1), 3Cl-HBA (40 μM for 48 h) or DMSO as control. RNA was extracted using the KAPA RiboErase RNA-Seq kit (Roche, Basel, Switzerland), and was analysed using the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) for quality control. The libraries were sequenced on an Illumina HiSeq X Ten platform.新生大鼠心肌细胞（Neonatal rat cardiomyocytes，NRCMs）经分离与培养后，以羟基羧酸受体1（Hydroxy-carboxylic Acid Receptor 1，HCAR1）的激动剂3Cl-HBA（40 μM 处理48 h）或二甲基亚砜（DMSO，作为对照）对其进行处理。采用KAPA RiboErase RNA测序试剂盒（KAPA RiboErase RNA-Seq kit，罗氏公司，瑞士巴塞尔）提取RNA，并通过安捷伦生物分析仪2100系统（Agilent Bioanalyzer 2100 system，安捷伦科技公司，美国加利福尼亚州）完成质量控制检测。随后，在Illumina HiSeq X Ten测序平台（Illumina HiSeq X Ten platform）上对构建的测序文库进行测序。