Inhibitory activity of selected pyrazolopyrimidine sulfamates in E1 enzyme assays.
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Homogeneous Time-Resolved Fluorescence (HTRF) enzyme assays were employed to evaluate the 50% Inhibitory Concentrations (IC50) values for compounds against Atg7 (autophagy-related protein-7), NAE (NEDD8-activating enzyme), UAE (ubiquitin activating enzyme) and SAE (SUMO-activating enzyme) with appropriately tagged ubiquitin-like proteins and E2 conjugating enzymes as described in the Methods. GABARAP = GABAA receptor-associated protein. Data represent mean ± SEM. n = Number of independent experiments.
创建时间：
2024-12-09
