Effect of loss of <i>MSH2</i> on frequencies of spontaneous DRR and translocation formation by HR following a DSB adjacent to one or both recombination substrates^a.

aMedian frequencies were determined from a minimum of 10 independent cultures for each strain. Fold differences from the median frequency observed with wild type strains are indicated in brackets. The 95% confidence intervals are indicated in parentheses. bFrequencies of DRR were determined with wild type and msh2::hisG mutant haploid strains. Frequencies of translocation formation were determined with homozygous wild type and msh2::hisG-URA3-hisG/msh2::hisG-URA3-hisG mutant diploid strains. cFrequencies of spontaneous DRR between 415 bp direct repeats at the HIS3 locus in haploid cells were determined as described in the Materials and Methods, and previously (75). dFrequencies of translocation formation in diploid cells by HR between a his3-Δ5′ substrate at the LEU2 locus on one copy of chromosome V and a his3-Δ3′ substrate at the HIS3 locus on one copy of chromosome XV were determined following a HO endonuclease-mediated break adjacent to the his3-Δ5′ substrate as described in the Materials and Methods, and previously (38). his3-Δ5′ substrates that shared either 60 bp or 300 bp of homology with the his3-Δ3′ substrate were used. The frequencies observed in wild type strains were reported previously (38). eFrequencies of translocation formation in diploid cells by HR between his3-Δ5′ and his3-Δ3′ following HO endonuclease-mediated break adjacent to both substrates were determined as described in the Materials and Methods, and previously (38). his3-Δ5′ substrates that shared either 60 bp or 300 bp of homology with the his3-Δ3′ substrate were used. The frequencies observed in both wild type msh2Δ/msh2Δ mutant strains were reported previously (38).