Mapping Nanoscale-to-Single Cell Phosphoproteomic Landscape by Chip-DIA

Mapping dynamic protein phosphorylation within trace samples deciphers cellular heterogeneity in signaling events and network activations for better understanding biology and disease mechanisms. We present a Chip-DIA strategy combining a microfluidic chip and data-independent acquisition mass spectrometry (DIA-MS) for sensitive nanoscale-to-single cell phosphoproteomic profiling.