Primers used for mutant complementation using vector pBAD33.
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aPrimers contain SmaI(bold) and HindIII(underlined), the PCR amplified product (1668 bp) was ligated to SmaI- HindIII digested pBAD33.bPrimers contain EcoRV(bold) and HindIII(underlined), the PCR amplified product (1559 bp) was ligated to SmaI- HindIII digested pBAD33.cPrimers contain SmaI(bold) and HindIII(underlined), the PCR amplified product (1267 bp) was ligated to SmaI- HindIII digested pBAD33.
创建时间：
2015-12-02
