毕节地区仿野生天麻性状和产量与质量数据集

数据获取和计算以下步骤：一是挑选各天麻资源下麻形良好、鹦哥嘴完整的成熟天麻块茎埋于沙壤土中，定期浇水，常温培养，保持湿度为40～50%左右，待其抽薹。二是天麻抽薹后，在天麻抽薹、开花、结果及成熟期分别调查其茎、花、果和块茎，按每平方米播种700 g零代种，常规管理，统计种植18个月后的单位面积产量及折干率。三是按2020年版中国药典天麻炮制要求将样品洗净蒸软，干燥处理，天麻素、对羟基苯甲醇含量测定参照张玥等的方法进行。四是待天麻抽薹完毕，将靠近茎段的较嫩部分用于提取DNA。五是回收得到的DNA片段可用琼脂糖凝胶电泳和紫外分光光度计检测浓度与纯度。六是选择7对引物开展天麻资源的SSR分析，选用1号、2号天麻DNA为模板，筛选引物。选择条带清晰、有差异带和重复性好的引物，用于全部天麻样本的分析。七是通过在电泳仪上电泳，之后在凝胶成像系统拍照，初步得到PCR电泳图，筛选多态性好的引物。八是采用中科瑞泰（北京）生物科技有限公司的DNA非变性PAGE电泳试剂盒，将SSR扩增产物在10%非变性聚丙烯酰胺凝胶上进行电泳检测。十是条带统计及聚类分析。

First, select mature Gastrodia elata tubers with good tuber shape and intact beak-shaped dormant buds (Yinggezui, a characteristic morphological structure of G. elata tubers), bury them in sandy loam soil, water regularly, culture at room temperature while maintaining humidity at approximately 40–50%, until the plants bolt.
Second, after G. elata bolts, investigate its stems, flowers, fruits and tubers at the bolting, flowering, fruiting and maturity stages respectively. Sow 700 g of zero-generation seeds per square meter, implement routine management, and count the yield per unit area and drying rate after 18 months of planting.
Third, wash and steam the samples until soft according to the processing requirements of Gastrodia elata specified in the 2020 Edition of Chinese Pharmacopoeia, followed by drying. The contents of gastrodin and p-hydroxybenzyl alcohol are determined referring to the method established by Zhang Yue et al.
Fourth, after all G. elata plants have completed bolting, collect the relatively tender parts adjacent to the stem segments for DNA extraction.
Fifth, detect the concentration and purity of the recovered DNA fragments using agarose gel electrophoresis and ultraviolet spectrophotometry.
Sixth, select 7 pairs of primers to perform Simple Sequence Repeats (SSR) analysis of G. elata resources. Use the DNA extracted from No.1 and No.2 G. elata as templates to screen primers, and select those with clear bands, differential bands and good reproducibility for the analysis of all G. elata samples.
Seventh, perform electrophoresis using an electrophoresis apparatus, then capture images with a gel imaging system to obtain preliminary PCR electropherograms, and screen primers with favorable polymorphism.
Eighth, use the DNA non-denaturing PAGE electrophoresis kit from Zhongkeruitai (Beijing) Biotechnology Co., Ltd. to conduct electrophoresis detection of SSR amplification products on 10% non-denaturing polyacrylamide gels.
Ninth, carry out band statistics and cluster analysis.

搜集汇总

背景与挑战

背景概述

该数据集由毕节市中药研究所自行产生，聚焦于仿野生天麻的性状、产量和质量数据，规模为40kb且无定期更新。其关键特点包括：通过标准化实验方法（如性状调查、成分测定和遗传分析）收集数据，支持植物育种、药物研发和农业管理等多领域应用，体现了数据的科学性和实用性。