Stability of the non-cleavable PEG6-C2-MMAD and PEG6-C2-Aur3377 conjugates under <i>in vitro</i> and <i>in vivo</i> conditions.

The percentage of intact MMAD was calculated as the ratio of treated DAR to untreated DAR. Degradation of the C-terminal portion of the PEG6-C2-MMAD payload is considered equivalent to payload removal for DAR determination purposes. Calculations are based on DAR values obtained from HIC analysis for most conjugates, except for Site G, H, and I conjugates for which the percentage of intact MMAD was determined by mass spec analysis. For mouse plasma stability, values reported are averages of three independent experiments. For mouse in vivo stability, samples collected from individually dosed mice were pooled to obtain the measurement. Stability of the non-cleavable PEG6-C2-MMAD and PEG6-C2-Aur3377 conjugates under in vitro and in vivo conditions.