Transcription profiling of mouse white adipose tissue from PPARb/d knockout

Analysis of white adipose tissue of PPARb/d knockout mice. Data may point towards putative target genes of PPARb/d and thus the function of PPARb/d in white adipose tissue. Datasets were used to identify glycogen synthase 2 as novel PPAR target. Experiment Overall Design: Three month old male PPARb/d knock-out mice were on a mixed background (Sv129/C57BL/6). Wild-type littermates served as control animals. Animals received standard chow. Epididymal white adipose tissue (WAT) was removed and total RNA was isolated and pooled afterwards (five animals per group). Pooled RNA was hybridized to Affymetrix mouse genome 430 2.0 arrays arrays. Five microgram total RNA was labeled according to the ENZO-protocol, fragmented and hybridized according to Affymetrix protocols.本研究针对过氧化物酶体增殖物激活受体β/δ（PPARb/d）敲除小鼠的白色脂肪组织（white adipose tissue, WAT）展开分析。本数据集旨在挖掘PPARb/d的潜在靶基因，以阐明其在白色脂肪组织中的生物学功能，曾被用于鉴定糖原合酶2（glycogen synthase 2）为新型PPAR靶标。 实验整体设计：选取3月龄雄性PPARb/d敲除小鼠，其遗传背景为Sv129/C57BL/6混合品系，以同窝野生型小鼠作为对照。所有实验动物均饲喂标准维持饲料。摘取附睾白色脂肪组织，提取总RNA并按组混合（每组5只小鼠）。将混合后的总RNA与Affymetrix小鼠基因组430 2.0芯片进行杂交：取5 μg总RNA，依照ENZO试剂盒流程完成标记与片段化，随后按照Affymetrix标准实验流程完成杂交。