RNA-seq analysis of epididymal adipose tissue from wild-type and Maf1 knockout mice

Purpose: To profile the effect of the Maf1 knockout on RNA polymerase II and RNA polymerase III transcriptomes in white adipose tissue Methods: Epididymal adipose tissue was harvested from overnight fasted 22-24 week old male mice that had been fed ad libitum on a chow diet. Total RNA was prepared from three biologically independent samples per genotype and subject to single-end directional RNA sequencing on the Illumina HiSeq platform. Sequence reads that passed quality filters were aligned against the Mm9 Mouse genome assembly using GSNAP and uniquely mapped reads were counted using HTseq-counts.研究目的：表征Maf1基因敲除对白色脂肪组织中RNA聚合酶II（RNA polymerase II）与RNA聚合酶III（RNA polymerase III）转录组的影响。 实验方法：选取以标准饲料（chow diet）自由采食、经整夜禁食的22~24周龄雄性小鼠，摘取其附睾脂肪组织（epididymal adipose tissue）。每个基因型设置3个生物学重复样本，提取总RNA后，在Illumina HiSeq测序平台上开展单端定向RNA测序（single-end directional RNA sequencing）。通过质量过滤（quality filters）的序列读数采用GSNAP比对至Mm9小鼠基因组组装（Mm9 Mouse genome assembly），并利用HTseq-counts对唯一比对读数（uniquely mapped reads）进行计数。