Summary of the defects observed upon mutations in She2p or She3p.

“Synergistic RNA binding” was assessed by EMSAs, She2p “RNA binding” by filter-binding assays, She3p “RNA binding” by EMSAs, and “She2p/She3p interaction” by pull-down experiments. All RNA binding experiments were performed with ASH1-E3-118 RNA. “+++” indicates wt-like, “++” moderately reduced, “+” reduced, and “—” abrogated binding. “n.d.” indicates that this experiment was not performed and (*) marks that the binding was wt-like in pull-down but reduced in size-exclusion chromatography experiments.