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Single cell RNA-seq of mouse lung endothelial cells

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Single cell RNA sequencing of endothelial cells isolated from adult Prox1-GFP mouse lung Lymphatic endothelial cells (PECAM1+ Prox1-GFP+ CD45- CD11b- Ter119-) and blood endothelial cells (PECAM1+ Prox1-GFP- CD45- CD11b- Ter119-) from adult Prox1-GFP mouse lung were sorted by flow cytometry into 384-well plates and subjected to single cell RNA-seq analysis by Smart-seq2.

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