Transcriptomes of whole blood and PBMC in chickens.

The gene expression profiles of chicken whole blood (WB) and peripheral blood mononuclear cells (PBMC) have been characterized and compared by microarray analysis. This study highlights the respective advantages of each blood cell subset and show that whole blood is more suitable for large scale studies and allows for the exploration of biological processes which are so far poorly investigated. Whole blood and PBMC from four male broilers (9 weeks old) were collected and isolated before -80°C conservation.