A549 and MDA-MB-231 cells were transfected with the Bloom-specific siRNA. Total RNA was extracted using Trizol at 48 hours after transfection. RNA-SEQ was carried out to profile the gene expression in
In the study presented here POSH2 was overexpressed in the MCF10AT breast cancer cells. RNA sequencing profiling of mRNA levels was performed in the control and overexpression cells.