A coronin 1-dependent signaling axis in T cells essential for allograft rejection

The goal of this analysis was to assess the similarity in transcriptomes between WT and Coro1-/- across regulatory and conventional T cells. mRNA profiles of wild-type and Coronin1A knockout from murine regulatory (trg) and conventional (con) T cells were generated by deep sequencing, in triplicate, using Illumina TruSeq stranded mRNA sample kit.本分析旨在评估野生型（wild-type, WT）与Coronin1A基因敲除（Coro1-/-）小鼠调节性T细胞和常规T细胞的转录组相似性。研究采用Illumina TruSeq链特异性mRNA样本制备试剂盒，通过深度测序对小鼠调节性（trg）与常规（con）T细胞的野生型及Coronin1A基因敲除样本完成了三次生物学重复的mRNA表达谱构建。