Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [Elav_ChR2]. Drosophila melanogaster

We identified ARGs in a genome-wide manner, in fly brains as well as in sorted neurons; they included dopaminergic neurons (DA) and a subset of circadian-related neurons (PDF+ neurons). Overall design: Elav-GAL4;UAS-ChR2-XXL or Elav-GAL4 flies stimulated with 30-second blue LED and mRNA isolated and sequenced 0, 15, 30, 60 min after.