Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Smad7-/- E15.5 tooth germ Transcriptomes

Smad7 is known to regulate both Smad-dependent and Smad-independent TGF-beta signaling pathways but is also extensively involved in crosstalks with other signaling pathways. To investigate the underlying molecular mechanism, we conducted RNA-Seq analysis on E15.5 molars from Smad7 mutant and control mice. Tooth germ mRNA profiles of E15.5 wild type (WT) and Smad7-/- mice were generated by deep sequencing, in triplicate, using Illumina NextSeq 550.Smad7 已知可同时调控Smad依赖型与Smad非依赖型转化生长因子-β（transforming growth factor-β, TGF-β）信号通路，且广泛参与与其他信号通路的交叉对话。为探究其潜在的分子机制，我们对Smad7敲除突变型与对照小鼠的E15.5胎龄磨牙开展了RNA测序（RNA-Seq）分析。本研究采用Illumina NextSeq 550平台进行三次生物学重复的深度测序，获取了E15.5胎龄野生型（wild type, WT）与Smad7敲除（Smad7-/-）小鼠的牙胚mRNA表达谱。