Exosomal microRNA sequencing in Tongxinluo pretreated and non-treated MSC-derived exosomes

In order to investigate the specific mechanisms underlying the cardioprotective effects of Tongxinluo pretreated MSC-derived exosomes (MSCTXL-Exo) in cardiac repair, we performed microRNA sequencing on exosomes secreted from Tongxinluo pretreated MSCs and non-treated MSCs to identify differentially expressed miRNA. We found that 18 miRNAs were identified to be upregulated and 25 miRNAs downregulated (over 2-fold change) in MSCTXL-Exo compared to MSC-Exo.为探究通心络预处理的间充质干细胞（Mesenchymal Stem Cell, MSC）来源外泌体（MSCTXL-Exo）在心脏修复中发挥心脏保护作用的具体机制，我们对通心络预处理的MSC及未处理MSC分泌的外泌体进行了微小RNA测序，以鉴定差异表达的miRNA。结果发现，相较于普通MSC来源外泌体（MSC-Exo），MSCTXL-Exo中存在18个上调表达的miRNA及25个下调表达的miRNA，其表达倍数变化超过2倍。