The ribosomal prolyl-hydroxylase OGFOD1 decreases during cardiac differentiation, modulates translation and spliceosomal processes

In this study we provide the gene expression profiles and MISO analyses for alternative splicing events such as exon skipping in wildtype versus OGFOD1 (oxoglutarate, glucose and iron dependent protein 1)-knock-out iPSC-derived cardiomyocytes, we further compare the profiles to wildtype which were treated with a drug inhibiting α-ketoglutarate-dependent hydroxylases (dimethyloxalylglycine) versus vehicle control. Altered translation and splicing processes play a role in differentiation processes and have been shown to be involved in disease progression in e.g. heart failure. OGFOD1 is a ribosomal prolyl-hydroxylase and which influences translation, here we show its further importance in cardiac differentiation and alternative splicing.

本研究提供了野生型与OGFOD1（oxoglutarate, glucose and iron dependent protein 1，氧戊二酸、葡萄糖及铁依赖蛋白1）敲除的诱导多能干细胞（induced pluripotent stem cell, iPSC）来源心肌细胞的基因表达谱，以及外显子跳跃等可变剪接事件的MISO分析结果；我们进一步将该表达谱与经α-酮戊二酸依赖型羟化酶抑制剂二甲基乙二酰甘氨酸（dimethyloxalylglycine）处理的野生型样本以及溶剂对照样本进行了对比分析。异常的翻译与剪接过程参与细胞分化进程，且已被证实与心力衰竭等疾病的进展密切相关。OGFOD1是一种核糖体脯氨酸羟化酶，可调控翻译过程；本研究进一步阐明了其在心脏分化与可变剪接调控中的重要作用。

搜集汇总

背景与挑战

背景概述

该数据集研究了核糖体脯氨酰羟化酶OGFOD1在心脏分化过程中的作用，通过RNA测序分析了人类诱导多能干细胞衍生的心肌细胞中的基因表达和选择性剪接事件。数据集包含12个样本，比较了OGFOD1敲除细胞与野生型细胞，以及使用药物抑制羟化酶的实验组，旨在揭示OGFOD1在翻译和剪接过程中的调控功能。