RNAseq of NRVMs infected with AdCryABR120G by the manipulation of Ube2v1 expression

Neonatal rat ventricle myocytes (NRVMs) were infected with CryABR120G adenovirus. Knockdown or overexpression of Ube2v1 was performed by siUbe2v1 transfection or AdUbe2v1 infection in CryABR120G-expressing cells. Total RNA was extracted from NRVMs of four groups: CTR (untreated control), R120G (AdCryABR120G infection), RSIV1 (AdCryABR120G infection with siUbe2v1 transfection), and RADV1 (coinfection with AdCryABR120G and AdUbe2v1), in triplicate, for gene expression profiling.本研究将新生大鼠心室肌细胞（Neonatal rat ventricle myocytes, NRVMs）以CryABR120G腺病毒（CryABR120G adenovirus）进行感染。在表达CryABR120G的细胞中，通过siUbe2v1转染或AdUbe2v1感染分别实现Ube2v1的敲低与过表达。随后，从四组NRVMs中提取总RNA，每组设置三次生物学重复以开展基因表达谱分析：四组分别为未处理对照组（CTR, untreated control）、AdCryABR120G感染组（R120G）、AdCryABR120G感染联合siUbe2v1转染组（RSIV1），以及AdCryABR120G与AdUbe2v1共感染组（RADV1）。