Detectability of swine influenza A virus RNA by qRT-PCR and virus isolation in nasal swab samples (A) and oral fluid samples (B) of pigs sequentially infected with swine influenza A strains sw/Gent/28/10 (H1N1) at day 0 and sw/Gent/172/08 (H3N2) at day 21.

ns: No sample available; +: PCR pos; -: PCR neg †: Samples with a Ct value < 30 submitted for virus isolation on embryonated chicken eggs (EVE) and MDCK cells ‡: Significant difference between swIAV detection rates in nasal swab samples and oral fluid at that particular time point (p<0,05) Ϯ: No measure of association is computed because both variables are constant 1: Samples analyzed by virus isolation on ECE with positive result 2: Samples analyzed by virus isolation on MDCK with positive result Detectability of swine influenza A virus RNA by qRT-PCR and virus isolation in nasal swab samples (A) and oral fluid samples (B) of pigs sequentially infected with swine influenza A strains sw/Gent/28/10 (H1N1) at day 0 and sw/Gent/172/08 (H3N2) at day 21.