Table 5 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS / MS data of reference desulfoglucosinolates

Table 5 Profiles of glucosinolates (GSLs) derived from aliphatic amino acids in various organs of Planodes virginica, Nasturtium officinale and Rorippa sylvestris. a Methylsulfinylalkyls (MeSOalkyls)MeSO2alkylsMethylthioalkyls (MeSalkyls)n = 4n = 5n = 6n = 7n = 8n = 9* n = 8n = 5*n = 6*n = 7n = 8n = 9*647267666968 *8094 *88 *8792[89] *μmol/g dry wt.Planodes virginica (Acc. 1, Kentucky)Rootstr.0.040.020.280.790.050.01*n.d.tr.*0.020.02n.d.Stemstr.0.010.040.280.780.050.01*n.d.n.d.n.d.n.d.n.d.Leavestr.0.010.020.180.480.050.01*n.d.n.d.n.d.n.d.n.d.Siliquestr.0.020.040.431.20.090.03*n.d.n.d.n.d.n.d.n.d.Seedstr.b0.10b0.384.8130.480.05*tr.tr.*0.481.3tr.*Planodes virginica (Acc. 2, Ohio)Leavestr.b0.040.201.21.70.110.02*n.d.n.d.n.d.n.d.n.d.Nasturtium officinaleSeeds (OH)tr.*0.020.093.93.90.040.17n.d.0.84.82.0n.d.Seeds (John)tr.*0.02b0.124.95.50.120.12n.d.1.55.12.5n.d.Rorippa sylvestrisSeedsn.d.n.d.n.d.0.06563.21.1n.d.n.d.0.02*100.05* a Data (mean levels) and N from the same collection as in Table 4. In addition to the listed GSLs, an unidentified isomer of 2h3mPe (149), with the desulfo derivative closely eluting yet chromatographically distinct from d 149, was detected at ca. 0.01 μmol/g dry wt. in P. virginica (both accessions), and unidentified isomers of 2mBu and 3mPe at trace level were detected in N. officinale (Section 2.2.4.). Searched for but not detected in any of the three species: 10mSOd, 10mSd, Pren, Buen, the typical A. thaliana GSLs 3hPr, 4hBu, 3BzOp, 4BzOb, 5BzOp, as well as BCAA-derived 1mEt, 1mPr, 2mPr, 2mBu, 2h2mBu, 3mPe, 3hmPe, 2h3mPe. GSL numbers labelled with an asterisk indicate tentative identification due to lack of an authentic standard, while individual data with an asterisk indicate lack of MS2 confirmation of analyte identity due to low level. b Identity confirmed by comp. witn an authentic std. using UHPLC-QToF (Supplementary Fig. S4F-G).