rDNA chromatin interactions in the MYC-driven Eμ-Myc transgenic mouse model of B-cell lymphoma [4C-seq]. Mus musculus

Long-range interactions of ribososomal DNA (rDNA) identified by 4C-seq in mouse wildtype, premalignant and malignant Eμ-Myc cells and tissue culture adapated Eμ-Myc-shUbtf and Eμ-Myc-LMP (empty vector) cells. Overall design: We performed 4C-seq in mouse wildtype, premalignant and malignant cells primary preB Eμ-Myc cells and tissue culture adapated Eμ-Myc-shUbtf and Eμ-Myc-LMP. For this we used multiple bait primers across the rDNA and identified long-range interactions in 5000bp windows. The interaction frequency, represented as read counts per window, was compared between wt, prem and mal cells and only interactions also identified in Eμ-Myc-shUbtf and Eμ-Myc-LMP used for further analysis.