RNAseq of Wildtype, Smchd1 KO, Dux KO, Double KO, Tet-TKO and quadurple KO ES cells

RNAseq was performed by to compare gene expression between wildtype and Smchd1 KO ES cells, the gene expression pattern in Dux KO mutants , Double KO mutant Tet-TKO mutants and Tet TKO plus SMCKHD1 KO mutants were analyzed by RNAseq. Overall design: Three biological replicates per genotype; RNA was isolated individually from each replicate; three samples for WT, Smchd1 KO, Dux KO, Double KO(Smchd1 plus Dux KO), Tet-TKO and quadKO(Tet-TKO plus Smchd1 K) were sequenced individually; three samples for WT, Smchd1 KO were sequeced two technical repilacate.