Characterizing role of striated-muscle specific ribosomal protein L3-like (Rpl3L) in mouse hearts

Overall goal: To elucidate the function served by Rpl3L in heart. Purpose of analysis: To determine effects of Rpl3L-deficiency on translational mRNA profile in heart. Experimental structure: Polysomes and monosomes were fractionated from cardiac ventricles and associated RNA extracted for library generation and differential gene expression analyses between wild-type and Rpl3L-null samples, using standard RNAseq pipeline.整体目标：阐明Rpl3L在心脏中的功能。分析目的：明确Rpl3L缺失对心脏翻译组mRNA表达谱的影响。实验设计：从心脏心室中分离多聚核糖体（polysomes）与单核糖体（monosomes），提取其结合的RNA以构建文库，并采用标准RNA测序（RNAseq）流程对野生型与Rpl3L基因敲除样本开展差异基因表达分析。