N6-methyladenosine (m6A) RNA methylation is the most abundant internal chemical modifications in eukaryotic messenger RNA (mRNA) as well as long non-coding RNA (lncRNA). Recently, m6A RNA methylation
M6A immunoprecipitation from 15min BrU labeled and fragmented nascent RNA. HEK293T cells were incubated with 2 mM bromouridine for 15 min labeling time (pulse). Whole-cell total RNA was extracted with
Complicating proteomic analysis of whole tissues is the obvious problem of cell heterogeneity in tissues, which often results in misleading or confusing molecular findings. Thus, the coupling of tissu
m6A MeRIP-seq was performed to investigate the divergent m6A modification patterns among different varieties of Oryza sativa at the transcriptome level.