Ψ-Atlas

Pseudouridine (Ψ) is a C5 glycoside isomer of uridine, formed by breaking the N1 glycosyl bond and undergoing a 180° base rotation. This modification stands as one of the most widespread post-transcriptional alterations in RNA and is universally distributed among diverse RNA species. This database encompasses 554,895 Ψ modification sites across various RNA categories, including mRNA, ncRNA, tRNA, rRNA, and others, in 78 distinct species reported in current literature. The sequencing methodologies employed comprise the next-generation sequencing techniques such as Ψ-Seq, Pseudo-Seq, CeU-Seq, PSI-Seq, RBS-Seq, HydraPsiSeq, BID-Seq, PRAISE-Seq, as well as third-generation sequencing methods like Direct RNA sequencing.假尿苷（Pseudouridine，Ψ）是尿苷的C5糖苷异构体，通过断裂N1糖苷键并发生180°碱基旋转而形成。该修饰是RNA中最为普遍的转录后修饰类型之一，广泛分布于各类RNA物种中。本数据库收录了当前文献报道的78个不同物种中，覆盖信使RNA（mRNA）、非编码RNA（ncRNA）、转运RNA（tRNA）、核糖体RNA（rRNA）等各类RNA的554,895个假尿苷修饰位点。所采用的测序技术包括下一代测序技术与第三代测序技术，其中下一代测序技术涵盖Ψ测序（Ψ-Seq）、伪测序（Pseudo-Seq）、CeU测序（CeU-Seq）、PSI测序（PSI-Seq）、RBS测序（RBS-Seq）、HydraPsi测序（HydraPsiSeq）、BID测序（BID-Seq）、PRAISE测序（PRAISE-Seq），第三代测序技术则包含直接RNA测序（Direct RNA sequencing）。

搜集汇总

背景与挑战

背景概述

Ψ-Atlas是一个专注于RNA假尿苷（Ψ）修饰的综合性数据库，假尿苷是RNA中普遍存在的转录后修饰。该数据库收录了554,895个假尿苷修饰位点，覆盖mRNA、ncRNA、tRNA、rRNA等多种RNA类型和20个物种，并整合了多种测序技术及分析工具。