Development of a glucoamylase hyper-production platform in thermophilic fungus Myceliophthora thermophila via the rational redesigning and engineering manipulation

The development of proteins hyper-production platform in thermophilic fungus Myceliophthora thermophila is essential for both molecular basis understanding and industrial process. Herein the glucoamylase hyper-production strain MtGM12 was generated from our previously strain MtYM6 via genetically engineering. Transcriptional profiling analyses revealed that the amylolytic gene expression levels were significantly up-regulated in the MtGM12 than in MtWT. mRNA profiles of Myceliophthora thermophila cells exposed to 1d starch and 2d starch.