splitmCherry system for observing FloA-SaeS interaction

NIAID Data Ecosystem2026-05-02 收录

蛋白质相互作用检测

荧光蛋白报告系统

数据链接：

https://data.mendeley.com/datasets/ssytzw6wpz 数据链接链接失效反馈

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资源简介：

mCherry were split into N and C terminal, ligated with FloA and SaeS respectively.

创建时间：

2025-01-17

Amplicon sequencing of BFG-PCA and BFG-Y2H libraries

蛋白质相互作用检测

DNA条形码

BFG-PCA and BFG-Y2H are methods utilizing synthetic DNA barcodes for detection of binary protein-protein interactions. Here we screened >11,000 bait/prey pairs to compare the interactions detected by

NIAID Data Ecosystem70

The inferred interaction status of S . cerevisiae paralogous pairs in PPI data detected exclusively by methods other than two-hybrid and pulldowns.

蛋白质相互作用检测

旁系同源对识别

(XLSX)

NIAID Data Ecosystem50

Systematic screening of SUMO regulated proteins in human cell with bimolecular fluorescence complementation(BiFC). Homo sapiens

SUMO化修饰筛选

蛋白质相互作用检测

In this project, we designed a SUMO-specific bimolecular fluorescence complementation screen framework, namely biFC-SUMO, to systematically identify the SUMO interaction proteins and SUMO-modified pro

NIAID Data Ecosystem30

Characterization of ultraID for PDB-MS

邻近标记技术比较

蛋白质相互作用检测

Comparison of BioID (24h labeling) and ultraID (10 min labeling) for PDB-MS (POI = Ago2, control protein = Rab11). 4 biological replicates per condition.

NIAID Data Ecosystem70

Statistically Inferring Protein−Protein Associations with Affinity Isolation LC−MS/MS Assays

蛋白质相互作用检测

高通量定量质谱

Affinity isolation of protein complexes followed by protein identification by LC−MS/MS is an increasingly popular approach for mapping protein interactions. However, systematic and random assay errors

Figshare2007-09-07 更新40

splitmCherry system for observing FloA-SaeS interaction

资源简介：

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