Mutations and corresponding amino acid substitutions in the mutant spectra FMDV R-Wt-4 and R-SSI-4 passaged in the presence of ribavirin^a.

aThe sequence of the 3D-coding region was determined for R-Wt-4 and R-SSI-4 (wild type and triple mutant FMDV) after 4 passages in the presence of 5000 µM ribavirin. The populations are those described in Figure 3 and Table 5. bMutation and deduced amino acid substitutions are relative to the sequence of the parental clone C-S8c1 [57]. Amino acid residues (single-letter code) are numbered from the N- to the C-terminus of 3D. Boldface type indicates a change in the amino acid residue. Procedures for nucleotide sequencing and identification of FMDV genomic regions are described in Materials and Methods. cNumber of different mutations found comparing the sequence of each individual clone with the corresponding sequence of FMDV C-S8c1 [57]. dNumber of G→A plus C→T substitutions found comparing the sequence of each individual clone with the corresponding sequence of FMDV C-S8c1 [57]. eThe percentage of synonymous and non-synonymous mutations is indicated in parenthesis.