七妹羊肚菌化学成分及其近缘种麦角甾醇的含量

The chemical constituents of Morchella eximia were isolated by modern chromatographic separation techniques, and the structures were elucidated by nuclear magnetic resonance (NMR), mass spectrometry (MS), etc. Subsequently, a HPLC method was established for determining the ergosterol content in the fruiting bodies of different species of morels. This method was applied to comparatively analyze the ergosterol content in different growth stages (juvenile, mature, and aging stages) of the fruiting bodies of three cultivated species of morels (Morchella importuna, M. sextelata and M. eximia). In total, 13 compounds were isolated and identified from the fruiting bodies of M. eximia, including 5 ergosterol-related compounds. Among these, compounds 2, 9, 10 and 12 were isolated from the genus Morchella for the first time. HPLC analysis revealed significant differences in ergosterol content in the fruiting bodies of different species and growth stages of morels. The highest content was found in the juvenile fruiting bodies of the three species [(1.647±0.013)-(2.289±0.011) mg/g], however the content comparatively decreased in the mature stage. M. sextelata had the highest content [(1.796± 0.008) mg/g], while M. importuna had the lowest [(1.250±0.004) mg/g] in the mature stage. These findings are significant for the further development, utilization, and quality evaluation of morels.