SCF -licensed induction of a mucosal mast cell-like transcriptional program by TGF-b

This study evaluates the effects of 72h stimulus with stem cell factor (SCF) (100 ng/mL) and TGF-b (1 ng/mL or 10 ng/mL), both alone and in combination, on the transcriptome of bone marrow-derived cultured mast cells (BMMCs) grown in IL-3 for 6-8 weeks. Through this approach, we find that SCF dramatically enhances TGF-b mediated upregulation of signature genes associated with mucosal mast cells, including transcripts encoding the proteases mMCP-1 (Mcpt1) and mMCP-2 (Mcpt2). Replicates indicate biologic duplicates. Samples were sequenced on an Illumina NextSeq 500. Overall design: RNA-seq analysis of bone marrow-derived cultured mast cells grown in IL3 (5 ng/mL) and stimulated with TGF-b, SCF, or the two factors in combination for 72 hours at the listed concentrations