Reduced MLH1 expression in 129 versus B6 mice.

Quantification of MLH1 (A) mRNA and (B, C) protein levels in the striatum of B6.Mlh1+/+, 129.Mlh1+/+ and B6.Mlh1+/− 10-week-old mice (n = 3). (A) Striatal Mlh1 mRNA levels (TaqMan Mm00503449_m1, exons 11–12) in 129.Mlh1+/+ mice were significantly reduced by approximately 50% when compared to B6.Mlh1+/+ (p<0.05), and were comparable to levels in B6.Mlh1+/− mice. (B, C) Western blot analysis of MLH1 protein revealed significantly reduced levels in 129.Mlh1+/+ striata compared to B6.Mlh1+/+ striata. Bar graphs represent mean ±SD. *, p<0.05; **, p<0.01.