Ventilation-induced alveolar derecruitment and dysorganisation of cell-cell contacts between alveolar epithelial cells in SP-B deficient mice

supplemental video 1: Micro-computed tomography of the right lung after 4 hours of low volume ZEEP ventilation before a deep inflation of a mouse assigned to ON-DI4 subgroup. Voxelsize: 6 µm. supplemental video 2: Micro-computed tomography of the right lung after 4 hours of low volume ZEEP ventilation before a deep inflation of a mouse assigned to ON-DI4 subgroup. Voxelsize: 5.88 µm. supplemental video 3: Focused-Ion Beam Scanning Electron Microscopy (FIB-SEM) of the cell-cell contacts between alveolar epithelial type 1 and type 2 cells of a mouse randomized to group ON-4DI. Based on this dataset, the model in Figure 8D was segmented. supplemental video 4A: Focused-Ion Beam Scanning Electron Microscopy (FIB-SEM) of the cell-cell contacts between alveolar epithelial type 1 and type 2 cells of a mouse randomized to group OFF-4DI. supplemental video 4B: Cropped dataset from 4A, focusing on the disorganized tight junction between alveolar epithelial type 1 and type 2 cells. Based on this dataset, the model in Figure 8B was segmented.