Targeted modulation of chicken genes in vitro using CRISPRa and CRISPRi toolkit . Targeted modulation of chicken genes in vitro using CRISPRa and CRISPRi toolkit

To investigate the effectiveness of CRISPR based gene modulation, IRF7 was repressed in dCas9-KRAB DF1 cell line or activated in dCas9-VPR DF1 cell line followed by whole transcriptome analysis Overall design: a. Differntiall gene expression analysis between mock (non-targeting) and IRF7 targeting gRNAs transfected dCas9-KRAB DF1 cells for assessing the endogenous targeted gene knockdown b. Differntiall gene expression analysis between mock (non-targeting) and IRF7 targeting gRNAs transfected dCas9-VPR DF1 cells for assessing the endogenous targeted gene activation