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Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction)

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NIAID Data Ecosystem2026-03-14 收录
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To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids.

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2023-03-20
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